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验证数据展示

  • WB analysis using 84729-4-RR

    WB analysis using 84729-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84729-4-RR (MECR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 84729-4-RR

    IHC staining of mouse heart using 84729-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 84729-4-RR

    IHC staining of mouse heart using 84729-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84729-4-RR

    Affinity and Kinetic Characterization of 84729-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84729-4-RR against Mouse MECR were performed. The affinity constant is below 1 pM.

MECR Recombinant antibody

MECR Recombinant Antibody for WB, IHC, ELISA
Cat No. 84729-4-RR
CloneNo. 242151C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

NRBF-1, Nrbf1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104, 2-enoyl thioester reductase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, mouse heart tissue, rat heart tissue, mouse stomach tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84729-4-RR targets MECR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MECR fusion protein Ag0465 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitochondrial trans-2-enoyl-CoA reductase
Synonyms NRBF-1, Nrbf1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104, 2-enoyl thioester reductase
Calculated Molecular Weight 40 kDa
Observed Molecular Weight37-40 kDa
GenBank Accession NumberBC003864
Gene Symbol MECR
Gene ID (NCBI) 26922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9DCS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial 2-enoyl-acyl-carrier protein reductase (MECR) is an enzyme in the mitochondrial fatty acid synthase (mtFAS) pathway. MECR is involved in the synthesis of lipoic acid and is required for mitochondrial respiratory competence. MECR is highly expressed in skeletal and heart muscles and exhibits lower expression in placenta, liver, kidney, and pancreas. It is weakly expressed or not expressed in the lung (PMID: 32440681, PMID: 37734847, PMID: 38296034).

实验方案

Product Specific Protocols
WB protocol for MECR antibody 84729-4-RRDownload protocol
IHC protocol for MECR antibody 84729-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols