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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84729-4-RR (same clone as 84729-4-PBS)

    WB analysis using 84729-4-RR (same clone as 84729-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84729-4-RR (MECR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

    IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

    IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84729-4-RR

    Affinity and Kinetic Characterization of 84729-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84729-4-RR against Mouse MECR were performed. The affinity constant is below 1 pM.

Mouse MECR Recombinant antibody, PBS Only

MECR Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84729-4-PBS
CloneNo. 242151C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

NRBF-1, Nrbf1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104, 2-enoyl thioester reductase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84729-4-PBS targets MECR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MECR fusion protein Ag0465 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitochondrial trans-2-enoyl-CoA reductase
Synonyms NRBF-1, Nrbf1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104, 2-enoyl thioester reductase
Calculated Molecular Weight 40 kDa
Observed Molecular Weight37-40 kDa
GenBank Accession NumberBC003864
Gene Symbol MECR
Gene ID (NCBI) 26922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9DCS3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial 2-enoyl-acyl-carrier protein reductase (MECR) is an enzyme in the mitochondrial fatty acid synthase (mtFAS) pathway. MECR is involved in the synthesis of lipoic acid and is required for mitochondrial respiratory competence. MECR is highly expressed in skeletal and heart muscles and exhibits lower expression in placenta, liver, kidney, and pancreas. It is weakly expressed or not expressed in the lung (PMID: 32440681, PMID: 37734847, PMID: 38296034).