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CDK5 Recombinant antibody

CDK5 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241908F10

Cat no : 84749-1-RR

Print datasheet

Synonyms

PSSALRE, EC:2.7.11.1, Cyclin-dependent-like kinase 5, Cyclin-dependent kinase 5, Cdk 5

验证数据展示

  • WB analysis using 84749-1-RR

    WB analysis using 84749-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 84749-1-RR

    IHC staining of rat brain using 84749-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5 antibody included
  • IHC staining of rat brain using 84749-1-RR

    IHC staining of rat brain using 84749-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5 antibody included

经过测试的应用

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells, mouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CDK5 Ready-To-Use IHC Kit. CDK5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84749-1-RR targets CDK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CDK5 fusion protein Ag28465 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin-dependent kinase 5
Synonyms PSSALRE, EC:2.7.11.1, Cyclin-dependent-like kinase 5, Cyclin-dependent kinase 5, Cdk 5
Calculated Molecular Weight 31 kDa, 33 kDa
Observed Molecular Weight 31-33 kDa
GenBank Accession NumberBC005115
Gene Symbol CDK5
Gene ID (NCBI) 1020
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ00535
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

实验方案

Product Specific Protocols
WB protocol for CDK5 antibody 84749-1-RRDownload protocol
IHC protocol for CDK5 antibody 84749-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols