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IARS2 Recombinant antibody

IARS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242219C5

Cat no : 84764-3-RR

Print datasheet

Synonyms

Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5

验证数据展示

  • WB analysis using 84764-3-RR

    WB analysis using 84764-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84764-3-RR

    WB analysis using 84764-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84764-3-RR

    IF Staining of HepG2 using 84764-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84764-3-RR

    Affinity and Kinetic Characterization of 84764-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84764-3-RR against Human IARS2 were performed. The affinity constant is 0.469 nM.

经过测试的应用

Positive WB detected inHeLa cells, mouse lung tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, rat heart tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IARS2 Ready-To-Use IHC Kit. IARS2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84764-3-RR targets IARS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IARS2 fusion protein Ag10696 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name isoleucyl-tRNA synthetase 2, mitochondrial
Synonyms Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5
Calculated Molecular Weight 1012 aa, 114 kDa
Observed Molecular Weight114 kDa
GenBank Accession NumberBC010218
Gene Symbol IARS2
Gene ID (NCBI) 55699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NSE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

实验方案

Product Specific Protocols
WB protocol for IARS2 antibody 84764-3-RRDownload protocol
IF protocol for IARS2 antibody 84764-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols