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验证数据展示

  • WB analysis using 84783-2-RR

    WB analysis using 84783-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84783-2-RR (FGD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84783-2-RR

    Affinity and Kinetic Characterization of 84783-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84783-2-RR against Human FGD1 were performed. The affinity constant is below 1 pM.

FGD1 Recombinant antibody

FGD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84783-2-RR

产品说明书

CloneNo. 242314C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSK-N-SH cells, U-87 MG cells, BxPC-3 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84783-2-RR targets FGD1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FGD1 fusion protein Ag3861 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FYVE, RhoGEF and PH domain containing 1
别名 Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY
计算分子量 901 aa, 91 kDa
观测分子量 130 kDa
GenBank蛋白编号BC034530
基因名称 FGD1
Gene ID (NCBI) 2245
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP98174
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).

实验方案

Product Specific Protocols
WB protocol for FGD1 antibody 84783-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols