• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84783-2-RR (same clone as 84783-2-PBS)

    WB analysis using 84783-2-RR (same clone as 84783-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84783-2-RR (FGD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84783-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01538-1

    Cytometric bead array standard curve of MP01538-1

    Cytometric bead array standard curve of MP01538-1, FGD1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84783-2-PBS. Detection antibody: 84783-1-PBS. Standard: Ag3861. Range: 6.25-200 ng/mL.

  • Affinity and Kinetic Characterization of 84783-2-RR

    Affinity and Kinetic Characterization of 84783-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84783-2-RR against Human FGD1 were performed. The affinity constant is below 1 pM.

FGD1 Recombinant antibody, PBS Only (Capture)

FGD1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84783-2-PBS

产品说明书

CloneNo. 242314C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84783-2-PBS targets FGD1 as part of a matched antibody pair:

MP01538-1: 84783-2-PBS capture and 84783-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 FGD1 fusion protein Ag3861 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FYVE, RhoGEF and PH domain containing 1
别名 Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY
计算分子量 901 aa, 91 kDa
观测分子量 130 kDa
GenBank蛋白编号BC034530
基因名称 FGD1
Gene ID (NCBI) 2245
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP98174
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).