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验证数据展示

  • WB analysis using 84792-5-RR

    WB analysis using 84792-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84792-5-RR (MCM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84792-5-RR

    IHC staining of human colon cancer using 84792-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM3 antibody included
  • FC experiment of HeLa using 84792-5-RR

    FC experiment of HeLa using 84792-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human colon cancer using 84792-5-RR

    IHC staining of human colon cancer using 84792-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM3 antibody included
  • Affinity and Kinetic Characterization of 84792-5-RR

    Affinity and Kinetic Characterization of 84792-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84792-5-RR against Human MCM3 were performed. The affinity constant is 73.2 pM.

MCM3 Recombinant antibody

MCM3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84792-5-RR

产品说明书

CloneNo. 242103B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Raji cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy MCM3 Ready-To-Use IHC Kit. MCM3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84792-5-RR targets MCM3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MCM3 fusion protein Ag7966 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 minichromosome maintenance complex component 3
别名 P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1
计算分子量 91 kDa
观测分子量 102-105 kDa
GenBank蛋白编号BC001626
基因名称 MCM3
Gene ID (NCBI) 4172
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP25205
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

实验方案

Product Specific Protocols
WB protocol for MCM3 antibody 84792-5-RRDownload protocol
IHC protocol for MCM3 antibody 84792-5-RRDownload protocol
FC protocol for MCM3 antibody 84792-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols