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SIRT7 Recombinant antibody

SIRT7 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

242167C9

Cat no : 84805-4-RR

Print datasheet

Synonyms

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

验证数据展示

  • WB analysis using 84805-4-RR

    WB analysis using 84805-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84805-4-RR (SIRT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 84805-4-RR

    IP experiment of HEK-293T using 84805-4-RR

    IP result of anti-SIRT7 (IP:84805-4-RR, 4ug; Detection:84805-4-RR 1:2000) with HEK-293T cells lysate 2200 ug.

  • Affinity and Kinetic Characterization of 84805-4-RR

    Affinity and Kinetic Characterization of 84805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84805-4-RR against Human SIRT7 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HCT 116 cells, Caco-2 cells, HEK-293T cells, rat liver tissue
Positive IP detected inHEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84805-4-RR targets SIRT7 in WB, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, rat
Immunogen SIRT7 fusion protein Ag31794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
Synonyms SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-
Calculated Molecular Weight45KD
Observed Molecular Weight 45 kDa
GenBank Accession NumberNM_016538
Gene Symbol SIRT7
Gene ID (NCBI) 51547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NRC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

实验方案

Product Specific Protocols
WB protocol for SIRT7 antibody 84805-4-RRDownload protocol
IP protocol for SIRT7 antibody 84805-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols