验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, HCT 116 cells, Caco-2 cells, HEK-293T cells, rat liver tissue |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84805-4-RR targets SIRT7 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | SIRT7 fusion protein Ag31794 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae) |
| 别名 | SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.- |
| 计算分子量 | 45KD |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | NM_016538 |
| 基因名称 | SIRT7 |
| Gene ID (NCBI) | 51547 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9NRC8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT7 antibody 84805-4-RR | Download protocol |
| IHC protocol for SIRT7 antibody 84805-4-RR | Download protocol |
| IP protocol for SIRT7 antibody 84805-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
