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Host
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验证数据展示

  • WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

    WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84805-4-RR (SIRT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

    IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

    IP result of anti-SIRT7 (IP:84805-4-RR, 4ug; Detection:84805-4-RR 1:2000) with HEK-293T cells lysate 2200 ug. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84805-4-RR

    Affinity and Kinetic Characterization of 84805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84805-4-RR against Human SIRT7 were performed. The affinity constant is below 1 pM.

SIRT7 Recombinant antibody, PBS Only

SIRT7 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 84805-4-PBS

产品说明书

CloneNo. 242167C9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IP, Indirect ELISA

别名

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84805-4-PBS targets SIRT7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 SIRT7 fusion protein Ag31794 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
别名 SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-
计算分子量45KD
观测分子量 45 kDa
GenBank蛋白编号NM_016538
基因名称 SIRT7
Gene ID (NCBI) 51547
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NRC8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.