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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

    WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84805-4-RR (SIRT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

    IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

    IP result of anti-SIRT7 (IP:84805-4-RR, 4ug; Detection:84805-4-RR 1:2000) with HEK-293T cells lysate 2200 ug. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84805-4-RR

    Affinity and Kinetic Characterization of 84805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84805-4-RR against Human SIRT7 were performed. The affinity constant is below 1 pM.

SIRT7 Recombinant antibody, PBS Only

SIRT7 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 84805-4-PBS
CloneNo. 242167C9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84805-4-PBS targets SIRT7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen SIRT7 fusion protein Ag31794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
Synonyms SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-
Calculated Molecular Weight45KD
Observed Molecular Weight 45 kDa
GenBank Accession NumberNM_016538
Gene Symbol SIRT7
Gene ID (NCBI) 51547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NRC8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.