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CD90.1 Recombinant antibody

CD90.1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241542D7

Cat no : 84832-1-RR

Print datasheet

Synonyms

CD90, Thy1, Thy 1, 241542D7

验证数据展示

  • WB analysis using 84832-1-RR

    WB analysis using 84832-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84832-1-RR (CD90.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84832-1-RR

    Affinity and Kinetic Characterization of 84832-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84832-1-RR against Mouse CD90.1 were performed. The affinity constant is 17.0 nM.

经过测试的应用

Positive WB detected inFVB mouse brain tissue, FVB mouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84832-1-RR targets CD90.1 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name thymus cell antigen 1, theta
Synonyms CD90, Thy1, Thy 1, 241542D7
Observed Molecular Weight25 kDa
Gene Symbol CD90
Gene ID (NCBI) 21838
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593).

实验方案

Product Specific Protocols
WB protocol for CD90.1 antibody 84832-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols