• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NUAK2 Recombinant antibody, PBS Only (Detector)

NUAK2 Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242220C1

Cat no : 84849-4-PBS

Print datasheet

Synonyms

OMPHK2, NUAK family SNF1-like kinase 2, NUAK 2, EC:2.7.11.1, ANPH2

验证数据展示

  • WB analysis using 84849-4-RR (same clone as 84849-4-PBS)

    WB analysis using 84849-4-RR (same clone as 84849-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84849-4-RR (NUAK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84849-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01626-3

    Sandwich ELISA standard curve of MP01626-3

    Sandwich ELISA standard curve of MP01626-3, Human NUAK2 Recombinant Matched Antibody Pair - PBS only. 84849-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35302. 84849-4-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

  • Sample test of MP01626-3

    Sample test of MP01626-3

    The mean NUAK2 concentration was determined to be 14.95 ng/mL in A549 cell extract based on a 2.3 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84849-4-RR

    Affinity and Kinetic Characterization of 84849-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84849-4-RR against Human NUAK2 were performed. The affinity constant is below 1 pM.

产品信息

84849-4-PBS targets NUAK2 as part of a matched antibody pair:

MP01626-3: 84849-2-PBS capture and 84849-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen NUAK2 fusion protein Ag35302 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NUAK family, SNF1-like kinase, 2
Synonyms OMPHK2, NUAK family SNF1-like kinase 2, NUAK 2, EC:2.7.11.1, ANPH2
Calculated Molecular Weight70kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_030952
Gene Symbol NUAK2
Gene ID (NCBI) 81788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H093
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUAK2, also known as OMPHK2 and SNARK, is the fourth member of the AMPK kinase family. The molecular mass of NUAK2 is 69 kDa and it plays a key role in neural tube closure during embryonic development (PMID: 32845958).