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验证数据展示

  • IF Staining of HepG2 using 84855-1-RR

    IF Staining of HepG2 using 84855-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84855-1-RR

    IF Staining of HeLa using 84855-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 84855-1-RR

    FC experiment of HepG2 using 84855-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FOXP4 Recombinant antibody (84855-1-RR, Clone:242356B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84855-1-RR

    Affinity and Kinetic Characterization of 84855-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84855-1-RR against Human FOXP4 were performed. The affinity constant is 0.402 nM.

FOXP4 Recombinant antibody

FOXP4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84855-1-RR

产品说明书

CloneNo. 242356B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Fork head-related protein-like A, FKHLA, 242356B4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FOXP4 Ready-To-Use IHC Kit. FOXP4 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84855-1-RR targets FOXP4 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FOXP4 fusion protein Ag10047 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box P4
别名 Fork head-related protein-like A, FKHLA, 242356B4
计算分子量 678 aa, 73 kDa
GenBank蛋白编号BC052803
基因名称 FOXP4
Gene ID (NCBI) 116113
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IVH2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.

实验方案

Product Specific Protocols
IF protocol for FOXP4 antibody 84855-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols