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验证数据展示

  • WB analysis of rat kidney using 84856-2-RR (same clone as 84856-2-PBS)

    WB analysis of rat kidney using 84856-2-RR (same clone as 84856-2-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84856-2-PBS in a different storage buffer formulation.

  • WB analysis of mouse kidney using 84856-2-RR (same clone as 84856-2-PBS)

    WB analysis of mouse kidney using 84856-2-RR (same clone as 84856-2-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84856-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01622-2

    Cytometric bead array standard curve of MP01622-2

    Cytometric bead array standard curve of MP01622-2, SLC3A1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84856-2-PBS. Detection antibody: 84856-3-PBS. Standard: Ag9525. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84856-2-RR

    Affinity and Kinetic Characterization of 84856-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84856-2-RR against Human SLC3A1 were performed. The affinity constant is below 1 pM.

SLC3A1 Recombinant antibody, PBS Only (Capture)

SLC3A1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84856-2-PBS

产品说明书

CloneNo. 242370D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1, Amino acid transporter heavy chain SLC3A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84856-2-PBS targets SLC3A1 as part of a matched antibody pair:

MP01622-2: 84856-2-PBS capture and 84856-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC3A1 fusion protein Ag9525 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
别名 D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1, Amino acid transporter heavy chain SLC3A1
计算分子量 685 aa, 79 kDa
观测分子量78-90 kDa
GenBank蛋白编号BC022386
基因名称 SLC3A1
Gene ID (NCBI) 6519
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07837
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)