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验证数据展示

  • WB analysis of HeLa using 84915-1-RR (same clone as 84915-1-PBS)

    WB analysis of HeLa using 84915-1-RR (same clone as 84915-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84915-1-RR (GPR56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84915-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01673-1

    Sandwich ELISA standard curve of MP01673-1

    Sandwich ELISA standard curve of MP01673-1, Human GPR56 Recombinant Matched Antibody Pair - PBS only. 84915-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2478. 84915-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01673-1

    Sample test of MP01673-1

    The mean GPR56 concentration was determined to be 5.01 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load and 5.18 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP01673-2

    Cytometric bead array standard curve of MP01673-2

    Cytometric bead array standard curve of MP01673-2, GPR56 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84915-1-PBS. Detection antibody: 84915-3-PBS. Standard: Eg2478. Range: 0.781-50 ng/mL.

  • Cytometric bead array standard curve of MP01673-1

    Cytometric bead array standard curve of MP01673-1

    Cytometric bead array standard curve of MP01673-1, GPR56 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84915-1-PBS. Detection antibody: 84915-2-PBS. Standard: Eg2478. Range: 0.781-50 ng/mL.

GPR56 Recombinant antibody, PBS Only (Capture)

GPR56 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84915-1-PBS

产品说明书

CloneNo. 242249A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

ADGRG1 N-terminal fragment, ADGRG1 NT, ADGRG1 C-terminal fragment, ADGRG1 CT, ADGRG1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84915-1-PBS targets GPR56 as part of a matched antibody pair:

MP01673-1: 84915-1-PBS capture and 84915-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01673-2: 84915-1-PBS capture and 84915-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 G protein-coupled receptor 56
别名 ADGRG1 N-terminal fragment, ADGRG1 NT, ADGRG1 C-terminal fragment, ADGRG1 CT, ADGRG1
计算分子量78 kDa
观测分子量65 kDa
GenBank蛋白编号NM_001145771.2
基因名称 GPR56
Gene ID (NCBI) 9289
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y653
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPR56 (G-protein-coupled receptor 56, also named ADGRG1) is a member of the adhesion G-protein coupled receptor (aGPCR) family and one of the important players in the normal development of the brain. It plays a pivotal role in diverse neurobiological processes, including cortical formation, oligodendrocyte development, and myelination (PMID: 32798453). Moreover, GPR56 has been reported to regulate VEGF secretion and angiogenesis in melanoma (PMID: 37579299).