验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells |
Planning an IHC experiment? We recommend our IHCeasy PRICKLE1 Ready-To-Use IHC Kit. PRICKLE1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84926-1-RR targets PRICKLE1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PRICKLE1 fusion protein Ag18407 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | prickle homolog 1 (Drosophila) |
| 别名 | RILP, REST/NRSF-interacting LIM domain protein 1, Prickle-like protein 1, EPM1B, 242506A8 |
| 计算分子量 | 831 aa, 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC114939 |
| 基因名称 | PRICKLE1 |
| Gene ID (NCBI) | 144165 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96MT3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRICKLE1 antibody 84926-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |