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验证数据展示

  • WB analysis of HeLa using 84937-1-RR (same clone as 84937-1-PBS)

    WB analysis of HeLa using 84937-1-RR (same clone as 84937-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84937-1-RR (Ninein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84937-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01704-2

    Sandwich ELISA standard curve of MP01704-2

    Sandwich ELISA standard curve of MP01704-2, Human Ninein Recombinant Matched Antibody Pair - PBS only. 84937-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3688. 84937-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL.

  • Sample test of MP01704-2

    Sample test of MP01704-2

    The mean Ninein concentration was determined to be 105.0 pg/mL in HeLa cell extract based on a 1.0 mg/mL extract load, 70.8 pg/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 76.3 pg/mL in NCCIT cell extract based on a 1.9 mg/mL extract load and 72.4 pg/mL in HT-29 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP01704-2

    Cytometric bead array standard curve of MP01704-2

    Cytometric bead array standard curve of MP01704-2, Ninein Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84937-3-PBS. Detection antibody: 84937-1-PBS. Standard: Ag3688. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84937-1-RR

    Affinity and Kinetic Characterization of 84937-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84937-1-RR against Human Ninein were performed. The affinity constant is 0.172 nM.

Ninein Recombinant antibody, PBS Only (Detector)

Ninein Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84937-1-PBS

产品说明书

CloneNo. 242525B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84937-1-PBS targets Ninein as part of a matched antibody pair:

MP01704-2: 84937-3-PBS capture and 84937-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Ninein fusion protein Ag3688 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ninein (GSK3B interacting protein)
别名 NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein
计算分子量 2090 aa, 243 kDa
观测分子量 240-255 kDa
GenBank蛋白编号BC034708
基因名称 Ninein
Gene ID (NCBI) 51199
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N4C6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.