• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84956-4-RR

    WB analysis using 84956-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84956-4-RR

    IF Staining of HeLa using 84956-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 84956-4-RR

    IF Staining of HepG2 using 84956-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RCN2 Recombinant antibody

RCN2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84956-4-RR

产品说明书

CloneNo. 242612A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55, Calcium binding protein ERC 55

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inrat liver tissue, HeLa cells, HEK-293 cells, U-251 cells, mouse liver tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RCN2 Ready-To-Use IHC Kit. RCN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84956-4-RR targets RCN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RCN2 fusion protein Ag0247 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 reticulocalbin 2, EF-hand calcium binding domain
别名 E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55, Calcium binding protein ERC 55
计算分子量 37 kDa
观测分子量 55 kDa
GenBank蛋白编号BC004892
基因名称 RCN2
Gene ID (NCBI) 5955
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14257
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

实验方案

Product Specific Protocols
WB protocol for RCN2 antibody 84956-4-RRDownload protocol
IF protocol for RCN2 antibody 84956-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols