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验证数据展示

  • WB analysis using 84967-1-RR

    WB analysis using 84967-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84967-1-RR (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 84967-1-RR

    FC experiment of Jurkat using 84967-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MEK1/2 Recombinant Antibody (84967-1-RR, Clone:242614G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Jurkat using 84967-1-RR

    IF Staining of Jurkat using 84967-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (84967-1-RR, Clone: 242614G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84967-1-RR

    Affinity and Kinetic Characterization of 84967-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84967-1-RR against Human MEK1/2 were performed. The affinity constant is below 1 pM.

MEK1/2 Recombinant antibody

MEK1/2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84967-1-RR

产品说明书

CloneNo. 242614G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A549 cells, mouse brain tissue, rat brain tissue, HeLa cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84967-1-RR targets MEK1/2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MEK1/2 fusion protein Ag1481 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 2
别名 MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC018645
基因名称 MEK2
Gene ID (NCBI) 5605
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36507
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 84967-1-RR can recognize both the MEK2 and MEK1.

实验方案

Product Specific Protocols
WB protocol for MEK1/2 antibody 84967-1-RRDownload protocol
IF protocol for MEK1/2 antibody 84967-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols