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验证数据展示

  • WB analysis using 84971-3-RR (same clone as 84971-3-PBS)

    WB analysis using 84971-3-RR (same clone as 84971-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84971-3-RR (GS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

    IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

    IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 84971-3-RR (same clone as 84971-3-PBS)

    IF Staining of NIH/3T3 using 84971-3-RR (same clone as 84971-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01719-2

    Cytometric bead array standard curve of MP01719-2

    Cytometric bead array standard curve of MP01719-2, GS28 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84971-3-PBS. Detection antibody: 84971-4-PBS. Standard: Ag9021. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84971-3-RR

    Affinity and Kinetic Characterization of 84971-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84971-3-RR against Human GS28 were performed. The affinity constant is 2.23 nM.

GS28 Recombinant antibody, PBS Only (Capture)

GS28 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84971-3-PBS

产品说明书

CloneNo. 242552G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

GOSR1, GOS-28, Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84971-3-PBS targets GS28 as part of a matched antibody pair:

MP01719-2: 84971-3-PBS capture and 84971-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GS28 fusion protein Ag9021 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi SNAP receptor complex member 1
别名 GOSR1, GOS-28, Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28
计算分子量28.6 kDa
观测分子量 28 kDa
GenBank蛋白编号BC012620
基因名称 GS28
Gene ID (NCBI) 9527
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95249
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)