验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85024-6-RR targets RECQL in IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RECQL fusion protein Ag6834 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RecQ protein-like (DNA helicase Q1-like) |
| 别名 | DNA helicase, RecQ like type 1, DNA dependent ATPase Q1, DNA 3'-5' helicase Q1, ATP-dependent DNA helicase Q1, ATP dependent DNA helicase Q1 |
| 计算分子量 | 73 kDa |
| GenBank蛋白编号 | BC001052 |
| 基因名称 | RECQL |
| Gene ID (NCBI) | 5965 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P46063 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RECQL, also known as RecQ-like helicase, is a member of the RecQ DNA helicase family. These enzymes play a crucial role in maintaining genomic integrity by participating in various DNA repair processes, including mismatch repair, nucleotide excision repair, and direct repair. It's reported that the low RECQL expression is strongly associated with poor prognosis in breast cancer. RECQL expression may be a useful marker in estimating the prognosis of breast cancer patients. (PMID: 29914420)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RECQL antibody 85024-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |