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Species Reactivity
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验证数据展示

  • WB analysis using 85075-1-RR

    WB analysis using 85075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85075-1-RR

    WB analysis using 85075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85075-1-RR

    Affinity and Kinetic Characterization of 85075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85075-1-RR against Human PTK7 were performed. The affinity constant is 2.60 nM.

PTK7 Recombinant antibody

PTK7 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85075-1-RR

Print datasheet

CloneNo. 242720A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, CCK4, CCK 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, C2C12 cells, PC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85075-1-RR targets PTK7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PTK7 fusion protein Ag12026 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PTK7 protein tyrosine kinase 7
Synonyms Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, CCK4, CCK 4
Calculated Molecular Weight 1070 aa, 118 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC071557
Gene Symbol PTK7
Gene ID (NCBI) 5754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13308
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is an atypical receptor tyrosine kinase that consists of an extracellular domain with seven immunoglobulin-like domains, a transmembrane domain, and a catalytically defective tyrosine kinase domain (PMID: 29867084). It is implicated in planar cell polarity and the Wnt canonical and non-canonical pathways (PMID: 24618420). Overexpression of PTK7 has been reported in a variety of tumors, such as cervical cancer, colon cancer, lung adenocarcinoma, acute myelogenous leukemia, and gastric cancer (PMID: 30944666).

实验方案

Product Specific Protocols
WB protocol for PTK7 antibody 85075-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols