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验证数据展示

  • WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85075-1-PBS in a different storage buffer formulation.

  • WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85075-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01800-3

    Sandwich ELISA standard curve of MP01800-3

    Sandwich ELISA standard curve of MP01800-3, Human PTK7/CCK4 Recombinant Matched Antibody Pair - PBS only. 85075-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12026. 85075-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP01800-3

    Sample test of MP01800-3

    The mean PTK7/CCK4 concentration was determined to be 66.39 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 66.69 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01800-1

    Cytometric bead array standard curve of MP01800-1

    Cytometric bead array standard curve of MP01800-1, PTK7/CCK4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85075-1-PBS. Detection antibody: 85075-2-PBS. Standard: Ag12026. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85075-1-RR

    Affinity and Kinetic Characterization of 85075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85075-1-RR against Human PTK7 were performed. The affinity constant is 2.60 nM.

PTK7 Recombinant antibody, PBS Only (Capture/Detector)

PTK7 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85075-1-PBS

产品说明书

CloneNo. 242720A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, CCK4, CCK 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85075-1-PBS targets PTK7 as part of a matched antibody pair:

MP01800-1: 85075-1-PBS capture and 85075-2-PBS detection (validated in Cytometric bead array)

MP01800-3: 85075-2-PBS capture and 85075-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 PTK7 fusion protein Ag12026 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTK7 protein tyrosine kinase 7
别名 Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, CCK4, CCK 4
计算分子量 1070 aa, 118 kDa
观测分子量140 kDa
GenBank蛋白编号BC071557
基因名称 PTK7
Gene ID (NCBI) 5754
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13308
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is an atypical receptor tyrosine kinase that consists of an extracellular domain with seven immunoglobulin-like domains, a transmembrane domain, and a catalytically defective tyrosine kinase domain (PMID: 29867084). It is implicated in planar cell polarity and the Wnt canonical and non-canonical pathways (PMID: 24618420). Overexpression of PTK7 has been reported in a variety of tumors, such as cervical cancer, colon cancer, lung adenocarcinoma, acute myelogenous leukemia, and gastric cancer (PMID: 30944666).