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验证数据展示

  • WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85075-1-PBS in a different storage buffer formulation.

  • WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85075-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01800-3

    Sandwich ELISA standard curve of MP01800-3

    Sandwich ELISA standard curve of MP01800-3, Human PTK7/CCK4 Recombinant Matched Antibody Pair - PBS only. 85075-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12026. 85075-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP01800-3

    Sample test of MP01800-3

    The mean PTK7/CCK4 concentration was determined to be 66.39 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 66.69 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01800-1

    Cytometric bead array standard curve of MP01800-1

    Cytometric bead array standard curve of MP01800-1, PTK7/CCK4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85075-1-PBS. Detection antibody: 85075-2-PBS. Standard: Ag12026. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85075-1-RR

    Affinity and Kinetic Characterization of 85075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85075-1-RR against Human PTK7 were performed. The affinity constant is 2.60 nM.

PTK7 Recombinant antibody, PBS Only (Capture/Detector)

PTK7 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85075-1-PBS

Print datasheet

CloneNo. 242720A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, CCK4, CCK 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

85075-1-PBS targets PTK7 as part of a matched antibody pair:

MP01800-1: 85075-1-PBS capture and 85075-2-PBS detection (validated in Cytometric bead array)

MP01800-3: 85075-2-PBS capture and 85075-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen PTK7 fusion protein Ag12026 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PTK7 protein tyrosine kinase 7
Synonyms Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, CCK4, CCK 4
Calculated Molecular Weight 1070 aa, 118 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC071557
Gene Symbol PTK7
Gene ID (NCBI) 5754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13308
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is an atypical receptor tyrosine kinase that consists of an extracellular domain with seven immunoglobulin-like domains, a transmembrane domain, and a catalytically defective tyrosine kinase domain (PMID: 29867084). It is implicated in planar cell polarity and the Wnt canonical and non-canonical pathways (PMID: 24618420). Overexpression of PTK7 has been reported in a variety of tumors, such as cervical cancer, colon cancer, lung adenocarcinoma, acute myelogenous leukemia, and gastric cancer (PMID: 30944666).