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验证数据展示

  • WB analysis using 85089-1-RR

    WB analysis using 85089-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85089-1-RR (ASIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85089-1-RR

    WB analysis using 85089-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85089-1-RR (ASIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ASIC1 Recombinant antibody

ASIC1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85089-1-RR

Print datasheet

CloneNo. 242749B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACCN2, ASIC, Amiloride-sensitive cation channel 2, neuronal, Acid-sensing ion channel 1, 242749B11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, mouse brain tissue, LNCaP cells, SH-SY5Y cells, rat brain tissue, mouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85089-1-RR targets ASIC1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ASIC1 fusion protein Ag25941 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name amiloride-sensitive cation channel 2, neuronal
Synonyms ACCN2, ASIC, Amiloride-sensitive cation channel 2, neuronal, Acid-sensing ion channel 1, 242749B11
Calculated Molecular Weight 60 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberNM_001095
Gene Symbol ASIC1
Gene ID (NCBI) 41
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78348
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASIC1(Acid-sensing ion channel 1), also named as ACCN2, BNaC2 or amiloride-sensitive cation channel 2, is a member of the ASICs family. ASICs, an H+-gated subgroup of the epithelial Na+ channel/degenerin (ENaC/DEG) superfamily, are widely expressed throughout the central and peripheral nervous system and triggered by increased extracellular acidification (PMID: 28518134, 27941930). ASIC1 is a key player in acidosis-induced tumor growth and invasiveness. Clinically, alterations of ASIC1 are associated with poor survival in breast cancer (PMID: 26686084).

实验方案

Product Specific Protocols
WB protocol for ASIC1 antibody 85089-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols