验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, Saos-2 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
85103-3-RR targets SUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | SUN2 fusion protein Ag16077 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | unc-84 homolog B (C. elegans) |
Synonyms | UNC84B, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B, KIAA0668 |
Calculated Molecular Weight | 717 aa, 80 kDa |
Observed Molecular Weight | 80-85 kDa |
GenBank Accession Number | BC111717 |
Gene Symbol | SUN2 |
Gene ID (NCBI) | 25777 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UH99 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).
实验方案
Product Specific Protocols | |
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WB protocol for SUN2 antibody 85103-3-RR | Download protocol |
IF protocol for SUN2 antibody 85103-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |