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验证数据展示

  • WB analysis using 85103-3-RR (same clone as 85103-3-PBS)

    WB analysis using 85103-3-RR (same clone as 85103-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 85103-3-RR (same clone as 85103-3-PBS)

    WB analysis of HeLa using 85103-3-RR (same clone as 85103-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • IF Staining of HEPG2 using 85103-3-RR (same clone as 85103-3-PBS)

    IF Staining of HEPG2 using 85103-3-RR (same clone as 85103-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUN2 antibody (85103-3-RR, Clone: 242533G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01849-2

    Cytometric bead array standard curve of MP01849-2

    Cytometric bead array standard curve of MP01849-2, SUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85103-3-PBS. Detection antibody: 85103-2-PBS. Standard: Ag16077. Range: 1.25-80 ng/mL.

  • Cytometric bead array standard curve of MP01849-1

    Cytometric bead array standard curve of MP01849-1

    Cytometric bead array standard curve of MP01849-1, SUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85103-3-PBS. Detection antibody: 85103-1-PBS. Standard: Ag16077. Range: 0.625-80 ng/mL.

SUN2 Recombinant antibody, PBS Only (Capture)

SUN2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85103-3-PBS
CloneNo. 242533G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

UNC84B, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B, KIAA0668

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

85103-3-PBS targets SUN2 as part of a matched antibody pair:

MP01849-1: 85103-3-PBS capture and 85103-1-PBS detection (validated in Cytometric bead array)

MP01849-2: 85103-3-PBS capture and 85103-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SUN2 fusion protein Ag16077 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name unc-84 homolog B (C. elegans)
Synonyms UNC84B, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B, KIAA0668
Calculated Molecular Weight 717 aa, 80 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC111717
Gene Symbol SUN2
Gene ID (NCBI) 25777
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UH99
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).