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验证数据展示

  • WB analysis using 85103-3-RR (same clone as 85103-3-PBS)

    WB analysis using 85103-3-RR (same clone as 85103-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 85103-3-RR (same clone as 85103-3-PBS)

    WB analysis of HeLa using 85103-3-RR (same clone as 85103-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 85103-3-RR (same clone as 85103-3-PBS)

    IHC staining of human colon using 85103-3-RR (same clone as 85103-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85103-3-RR (SUN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • IF Staining of HEPG2 using 85103-3-RR (same clone as 85103-3-PBS)

    IF Staining of HEPG2 using 85103-3-RR (same clone as 85103-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUN2 antibody (85103-3-RR, Clone: 242533G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85103-3-RR (same clone as 85103-3-PBS)

    FC experiment of HeLa using 85103-3-RR (same clone as 85103-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SUN2 Recombinant antibody (85103-3-RR, Clone:242533G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01849-2

    Cytometric bead array standard curve of MP01849-2

    Cytometric bead array standard curve of MP01849-2, SUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85103-3-PBS. Detection antibody: 85103-2-PBS. Standard: Ag16077. Range: 1.25-80 ng/mL.

  • Cytometric bead array standard curve of MP01849-1

    Cytometric bead array standard curve of MP01849-1

    Cytometric bead array standard curve of MP01849-1, SUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85103-3-PBS. Detection antibody: 85103-1-PBS. Standard: Ag16077. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 85103-3-RR

    Affinity and Kinetic Characterization of 85103-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85103-3-RR against Human SUN2 were performed. The affinity constant is 23.7 pM.

SUN2 Recombinant antibody, PBS Only (Capture)

SUN2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 85103-3-PBS

产品说明书

CloneNo. 242533G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

UNC84B, 242533G3, FRIGG, KIAA0668, Protein unc-84 homolog B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85103-3-PBS targets SUN2 as part of a matched antibody pair:

MP01849-1: 85103-3-PBS capture and 85103-1-PBS detection (validated in Cytometric bead array)

MP01849-2: 85103-3-PBS capture and 85103-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 SUN2 fusion protein Ag16077 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 unc-84 homolog B (C. elegans)
别名 UNC84B, 242533G3, FRIGG, KIAA0668, Protein unc-84 homolog B
计算分子量 717 aa, 80 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC111717
基因名称 SUN2
Gene ID (NCBI) 25777
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UH99
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).