• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85103-3-RR

    WB analysis using 85103-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 85103-3-RR

    WB analysis of HeLa using 85103-3-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 85103-3-RR

    IHC staining of human colon using 85103-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85103-3-RR (SUN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEPG2 using 85103-3-RR

    IF Staining of HEPG2 using 85103-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUN2 antibody (85103-3-RR, Clone: 242533G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 85103-3-RR

    FC experiment of HeLa using 85103-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SUN2 Recombinant antibody (85103-3-RR, Clone:242533G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85103-3-RR

    Affinity and Kinetic Characterization of 85103-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85103-3-RR against Human SUN2 were performed. The affinity constant is 23.7 pM.

SUN2 Recombinant antibody

SUN2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 85103-3-RR

产品说明书

CloneNo. 242533G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

UNC84B, 242533G3, FRIGG, KIAA0668, Protein unc-84 homolog B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, Saos-2 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85103-3-RR targets SUN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SUN2 fusion protein Ag16077 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 unc-84 homolog B (C. elegans)
别名 UNC84B, 242533G3, FRIGG, KIAA0668, Protein unc-84 homolog B
计算分子量 717 aa, 80 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC111717
基因名称 SUN2
Gene ID (NCBI) 25777
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UH99
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).

实验方案

Product Specific Protocols
WB protocol for SUN2 antibody 85103-3-RRDownload protocol
IHC protocol for SUN2 antibody 85103-3-RRDownload protocol
IF protocol for SUN2 antibody 85103-3-RRDownload protocol
FC protocol for SUN2 antibody 85103-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols