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验证数据展示

  • WB analysis using 85121-4-RR

    WB analysis using 85121-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85121-4-RR (ADIPOR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 85121-4-RR

    IP experiment of mouse liver using 85121-4-RR

    IP result of anti-Adiponectin receptor (IP:85121-4-RR, 4ug; Detection:85121-4-RR 1:2000) with mouse liver tissue lysate 2400 ug.

  • IHC staining of mouse liver using 85121-4-RR

    IHC staining of mouse liver using 85121-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 85121-4-RR

    IHC staining of rat liver using 85121-4-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 85121-4-RR

    IF Staining of mouse liver using 85121-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (85121-4-RR, Clone: 242777F3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85121-4-RR

    Affinity and Kinetic Characterization of 85121-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85121-4-RR against Human Adiponectin receptor were performed. The affinity constant is 2.85 nM.

Adiponectin receptor Recombinant antibody

Adiponectin receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 85121-4-RR

产品说明书

CloneNo. 242777F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA

别名

ADIPOR2, ADIPOR1, Adiponectin receptor protein 2, adiponectin receptor 2, ACDCR2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, L02 cells, rat uterus tissue, human placenta tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85121-4-RR targets Adiponectin receptor in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Adiponectin receptor fusion protein Ag5744 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adiponectin receptor 2
别名 ADIPOR2, ADIPOR1, Adiponectin receptor protein 2, adiponectin receptor 2, ACDCR2
计算分子量 44 kDa
观测分子量45 kDa
GenBank蛋白编号BC051858
基因名称 Adiponectin receptor
Gene ID (NCBI) 79602
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86V24
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.

实验方案

Product Specific Protocols
WB protocol for Adiponectin receptor antibody 85121-4-RRDownload protocol
IHC protocol for Adiponectin receptor antibody 85121-4-RRDownload protocol
IF protocol for Adiponectin receptor antibody 85121-4-RRDownload protocol
IP protocol for Adiponectin receptor antibody 85121-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols