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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85121-4-RR (same clone as 85121-4-PBS)

    WB analysis using 85121-4-RR (same clone as 85121-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85121-4-RR (ADIPOR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    IP experiment of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    IP result of anti-Adiponectin receptor (IP:85121-4-RR, 4ug; Detection:85121-4-RR 1:2000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    IHC staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 85121-4-RR (same clone as 85121-4-PBS)

    IHC staining of rat liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    IF Staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (85121-4-RR, Clone: 242777F3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85121-4-RR

    Affinity and Kinetic Characterization of 85121-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85121-4-RR against Human Adiponectin receptor were performed. The affinity constant is 2.85 nM.

Adiponectin receptor Recombinant antibody, PBS Only

Adiponectin receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 85121-4-PBS

产品说明书

CloneNo. 242777F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

ADIPOR2, ADIPOR1, Adiponectin receptor protein 2, adiponectin receptor 2, ACDCR2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85121-4-PBS targets Adiponectin receptor in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Adiponectin receptor fusion protein Ag5744 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adiponectin receptor 2
别名 ADIPOR2, ADIPOR1, Adiponectin receptor protein 2, adiponectin receptor 2, ACDCR2
计算分子量 44 kDa
观测分子量45 kDa
GenBank蛋白编号BC051858
基因名称 Adiponectin receptor
Gene ID (NCBI) 79602
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86V24
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.