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验证数据展示

  • WB analysis using 85157-4-RR

    WB analysis using 85157-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85157-4-RR (DNAJC7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85157-4-RR

    IHC staining of mouse brain using 85157-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85157-4-RR (DNAJC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 85157-4-RR

    IF Staining of HepG2 using 85157-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJC7 antibody (85157-4-RR, Clone: 242875B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 85157-4-RR

    FC experiment of HeLa using 85157-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNAJC7 Recombinant antibody (85157-4-RR, Clone:242875B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85157-4-RR

    Affinity and Kinetic Characterization of 85157-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85157-4-RR against Human DNAJC7 were performed. The affinity constant is 0.767 nM.

DNAJC7 Recombinant antibody

DNAJC7 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 85157-4-RR

产品说明书

CloneNo. 242875B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

242875B3, CCRP, DANJC7, DNAJC 7, TPR2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, HEK-293 cells, Jurkat cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85157-4-RR targets DNAJC7 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNAJC7 fusion protein Ag1572 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 7
别名 242875B3, CCRP, DANJC7, DNAJC 7, TPR2
计算分子量 56 kDa
观测分子量56 kDa
GenBank蛋白编号BC011837
基因名称 DNAJC7
Gene ID (NCBI) 7266
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99615
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.

实验方案

Product Specific Protocols
WB protocol for DNAJC7 antibody 85157-4-RRDownload protocol
IHC protocol for DNAJC7 antibody 85157-4-RRDownload protocol
IF protocol for DNAJC7 antibody 85157-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols