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验证数据展示

  • WB analysis using 85161-4-RR

    WB analysis using 85161-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85161-4-RR (NUP214 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 85161-4-RR

    IHC staining of mouse kidney using 85161-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUP214 antibody included

NUP214 Recombinant antibody

NUP214 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85161-4-RR

产品说明书

CloneNo. 242782H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

242782H2, CAIN, CAN, KIAA0023

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, HEK-293T cells, MCF-7 cells, A431 cells, NIH/3T3 cells
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NUP214 Ready-To-Use IHC Kit. NUP214 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85161-4-RR targets NUP214 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NUP214 fusion protein Ag21377 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleoporin 214kDa
别名 242782H2, CAIN, CAN, KIAA0023
计算分子量 2080 aa, 213 kDa
观测分子量250 kDa
GenBank蛋白编号BC105998
基因名称 NUP214
Gene ID (NCBI) 8021
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35658
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473).

实验方案

Product Specific Protocols
WB protocol for NUP214 antibody 85161-4-RRDownload protocol
IHC protocol for NUP214 antibody 85161-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols