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验证数据展示

  • WB analysis using 85161-4-RR (same clone as 85161-4-PBS)

    WB analysis using 85161-4-RR (same clone as 85161-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85161-4-RR (NUP214 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 85161-4-RR (same clone as 85161-4-PBS)

    IHC staining of mouse kidney using 85161-4-RR (same clone as 85161-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP214 antibody included

NUP214 Recombinant antibody, PBS Only

NUP214 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85161-4-PBS

产品说明书

CloneNo. 242782H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

242782H2, CAIN, CAN, KIAA0023

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NUP214 Ready-To-Use IHC Kit. NUP214 primary antibody included.

产品信息

85161-4-PBS targets NUP214 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NUP214 fusion protein Ag21377 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleoporin 214kDa
别名 242782H2, CAIN, CAN, KIAA0023
计算分子量 2080 aa, 213 kDa
观测分子量250 kDa
GenBank蛋白编号BC105998
基因名称 NUP214
Gene ID (NCBI) 8021
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35658
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473).