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验证数据展示

  • FC experiment of HeLa using 85177-3-RR (same clone as 85177-3-PBS)

    FC experiment of HeLa using 85177-3-RR (same clone as 85177-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HUWE1 Recombinant antibody (85177-3-RR, Clone:242702F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

  • WB analysis using 85177-3-RR (same clone as 85177-3-PBS)

    WB analysis using 85177-3-RR (same clone as 85177-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85177-3-RR (HUWE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01895-2

    Cytometric bead array standard curve of MP01895-2

    Cytometric bead array standard curve of MP01895-2, HUWE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85177-1-PBS. Detection antibody: 85177-3-PBS. Standard: Ag13763. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 85177-3-RR

    Affinity and Kinetic Characterization of 85177-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85177-3-RR against Human HUWE1 were performed. The affinity constant is 26.1 nM.

HUWE1 Recombinant antibody, PBS Only (Detector)

HUWE1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 85177-3-PBS

产品说明书

CloneNo. 242702F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HUWE1 Ready-To-Use IHC Kit. HUWE1 primary antibody included.

产品信息

85177-3-PBS targets HUWE1 as part of a matched antibody pair:

MP01895-2: 85177-1-PBS capture and 85177-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HUWE1 fusion protein Ag13763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HECT, UBA and WWE domain containing 1
别名 242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1
计算分子量 437 aa, 482 kDa
观测分子量482 kDa
GenBank蛋白编号BC002602
基因名称 HUWE1
Gene ID (NCBI) 10075
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z6Z7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).