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验证数据展示

  • WB analysis using 85205-1-RR (same clone as 85205-1-PBS)

    WB analysis using 85205-1-RR (same clone as 85205-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85205-1-RR (CCKAR-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 85205-1-RR (same clone as 85205-1-PBS)

    IP experiment of mouse lung using 85205-1-RR (same clone as 85205-1-PBS)

    IP result of anti-CCKAR-specific (IP:85205-1-RR, 4ug; Detection:85205-1-RR 1:600) with mouse lung tissue lysate 1520 ug. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01958-1

    Sandwich ELISA standard curve of MP01958-1

    Sandwich ELISA standard curve of MP01958-1, Human CCKAR Recombinant Matched Antibody Pair - PBS only. 85205-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00105. 85205-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01958-1

    Sample test of MP01958-1

    The mean CCKAR concentration was determined to be 4.0 ng/mL in HUVEC cell extract based on a 0.9 mg/mL extract load and 5.3 ng/mL in SGC-7901 cell extract based on a 2.4 mg/mL extract load.

CCKAR-specific Recombinant antibody, PBS Only (Detector)

CCKAR-specific Uni-rAbTM Recombinant Antibody for WB, IP, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85205-1-PBS

产品说明书

CloneNo. 242681A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Sandwich ELISA, Indirect ELISA, Sample test

别名

CCKAR, CCK1-R, CCK1 R, CCK AR, CCK A receptor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85205-1-PBS targets CCKAR-specific as part of a matched antibody pair:

MP01958-1: 85205-2-PBS capture and 85205-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IP, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cholecystokinin A receptor
别名 CCKAR, CCK1-R, CCK1 R, CCK AR, CCK A receptor
计算分子量 48 kDa
观测分子量75 kDa
GenBank蛋白编号NM_000730
基因名称 CCKAR
Gene ID (NCBI) 886
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP32238
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification.