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验证数据展示

  • WB analysis using 85419-4-RR

    WB analysis using 85419-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85419-4-RR

    WB analysis using 85419-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85419-4-RR

    IF Staining of HepG2 using 85419-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85419-4-RR

    Affinity and Kinetic Characterization of 85419-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85419-4-RR against Human PTGES2 were performed. The affinity constant is 0.973 nM.

PTGES2 Recombinant antibody

PTGES2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85419-4-RR

产品说明书

CloneNo. 242930B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, HepG2 cells, HEK-293 cells, Jurkat cells, rat heart tissue, pig heart tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85419-4-RR targets PTGES2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PTGES2 fusion protein Ag1329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prostaglandin E synthase 2
别名 242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2
计算分子量 42 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC011613
基因名称 PTGES2
Gene ID (NCBI) 80142
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H7Z7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).

实验方案

Product Specific Protocols
WB protocol for PTGES2 antibody 85419-4-RRDownload protocol
IF protocol for PTGES2 antibody 85419-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols