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Host
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Conjugate
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验证数据展示

  • WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

    WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

  • WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

    WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85419-4-RR (same clone as 85419-4-PBS)

    IF Staining of HepG2 using 85419-4-RR (same clone as 85419-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85419-4-RR

    Affinity and Kinetic Characterization of 85419-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85419-4-RR against Human PTGES2 were performed. The affinity constant is 0.973 nM.

PTGES2 Recombinant antibody, PBS Only

PTGES2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85419-4-PBS

产品说明书

CloneNo. 242930B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, Indirect ELISA

别名

242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85419-4-PBS targets PTGES2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PTGES2 fusion protein Ag1329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prostaglandin E synthase 2
别名 242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2
计算分子量 42 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC011613
基因名称 PTGES2
Gene ID (NCBI) 80142
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H7Z7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).