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Anti-Human CD226 Rabbit Recombinant Antibody

CD226 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240673G4

Cat no : 98002-2-RR

Print datasheet

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

验证数据展示

  • FC experiment of human PBMCs using 98002-2-RR

    FC experiment of human PBMCs using 98002-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-2-RR, Clone: 240673G4) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98002-2-RR

    FC experiment of human PBMCs using 98002-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-2-RR, Clone:240673G4) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98002-2-RR

    Affinity and Kinetic Characterization of 98002-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98036-1-RR against Human CD226 were performed. The affinity constant is 3.70 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98002-2-RR is CD226 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD226 molecule
Synonyms DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen
Calculated Molecular Weight 336 aa, 39 kDa
GenBank Accession NumberBC074787
Gene Symbol CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15762
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

实验方案

Product Specific Protocols
FC protocol for CD226 antibody 98002-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols