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Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody

B7-H3/CD276 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230305A10

Cat no : 98017-1-RR

Print datasheet

Synonyms

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

验证数据展示

  • FC experiment of human monocyte-derived immature dendritic cells using 98017-1-RR

    FC experiment of human monocyte-derived immature dendritic cells using 98017-1-RR

    1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody (98017-1-RR, Clone: 230305A10) (red) or 0.25 ug Isotype Control(blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98017-1-RR

    Affinity and Kinetic Characterization of 98017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98017-1-RR against Human B7-H3/CD276 were performed. The affinity constant is 0.203 nM.

经过测试的应用

Positive FC detected inhuman monocyte-derived immature dendritic cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98017-1-RR is B7-H3/CD276 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD276 molecule
Synonyms CD276, B7-H3, B7H3, B7 homolog 3, B7 H3
Calculated Molecular Weight57 kDa
GenBank Accession NumberNM_001024736
Gene Symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5ZPR3
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

实验方案

Product Specific Protocols
FC protocol for B7-H3/CD276 antibody 98017-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols