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Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody, PBS Only

CD126/IL-6R alpha Uni-rAbTM Recombinant Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230371A7

Cat no : 98024-1-PBS

Print datasheet

Synonyms

Il6ra, CD126, 230371A7, IL-6R alpha, Il6r

验证数据展示

  • FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody (98024-1-RR, Clone: 230371A7) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed. This data was developed using the same antibody clone with 98024-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody (98024-1-RR, Clone: 230371A7) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98024-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00182-2

    Sandwich ELISA standard curve of MP00182-2

    Sandwich ELISA standard curve of MP00182-2, Mouse CD126/IL-6R alpha Monoclonal Matched Antibody Pair - PBS only. 98024-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0664. 83209-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Sample test of MP00182-2

    Sample test of MP00182-2

    J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. An aliquot of the cell culture supernatants were removed, assayed for mouse IL-6R alpha, and measured 402.5 pg/mL. Liver tissue from one mouse was homogenized and seeded into 100 mL of RPMI containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 50 μM β-mercaptoethanol for 3 days. An aliquot of the cell culture supernate was removed, assayed for mouse IL-6R alpha, and measured 345.1 pg/mL.

  • Sample test of MP00182-2

    Sample test of MP00182-2

    Serum of sixteen mice were measured. The IL-6R alpha concentration of detected samples was determined to be 10,180.3 pg/mL with a range of 6,065.2-15,362.2 pg/mL.

  • Affinity and Kinetic Characterization of 98024-1-RR

    Affinity and Kinetic Characterization of 98024-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98024-1 -RR against Mouse IL-6R alpha were performed. The affinity constant is 0.629 nM.

产品信息

98024-1-PBS targets CD126/IL-6R alpha as part of a matched antibody pair:

MP00182-2: 98024-1-PBS capture and 83209-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 receptor, alpha
Synonyms Il6ra, CD126, 230371A7, IL-6R alpha, Il6r
Calculated Molecular Weight50KD
GenBank Accession NumberNM_010559.3
Gene Symbol Il6ra
Gene ID (NCBI) 16194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22272
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).