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Anti-Human CD79a Rabbit Recombinant Antibody, PBS Only

CD79a Uni-rAbTM Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241299F3

Cat no : 98072-1-PBS

Print datasheet

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

验证数据展示

  • FC experiment of human PBMCs using 98072-1-RR (same clone as 98072-1-PBS)

    FC experiment of human PBMCs using 98072-1-RR (same clone as 98072-1-PBS)

    1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD19 (HIB19) (CL647-65110, Clone: HIB19) and 0.25 ug Anti-Human CD79a Rabbit Recombinant Antibody (98072-1-RR, Clone:241299F3), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98072-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98072-1-RR (same clone as 98072-1-PBS)

    FC experiment of human PBMCs using 98072-1-RR (same clone as 98072-1-PBS)

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD79a Rabbit Recombinant Antibody (98072-1-RR, Clone:241299F3)(red), or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98072-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01111-3

    Cytometric bead array standard curve of MP01111-3

    Cytometric bead array standard curve of MP01111-3, CD79A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98072-1-PBS. Detection antibody: 84236-2-PBS. Standard: RP02315. Range: 0.078-10 ng/mL.

  • Affinity and Kinetic Characterization of 98072-1-RR

    Affinity and Kinetic Characterization of 98072-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98072-1-RR against Human CD79a were performed. The affinity constant is 0.151 nM.

Planning an IHC experiment? We recommend our IHCeasy CD79a Ready-To-Use IHC Kit. CD79a primary antibody included.

产品信息

98072-1-PBS targets CD79a as part of a matched antibody pair:

MP01111-3: 98072-1-PBS capture and 84236-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD79a molecule, immunoglobulin-associated alpha
Synonyms MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain
Calculated Molecular Weight 226 aa, 25 kDa
GenBank Accession NumberBC113733
Gene Symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP11912
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.