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Anti-Human IL-1RAP Rabbit Recombinant Antibody, PBS Only

IL-1RAP Uni-rAbTM Recombinant Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240756A12

Cat no : 98073-1-PBS

Print datasheet

Synonyms

IL1RAP, IL 1RAcP, IL 1R3, IL 1R 3, EC:3.2.2.6

验证数据展示

  • FC experiment of human PBMCs using 98073-1-RR (same clone as 98073-1-PBS)

    FC experiment of human PBMCs using 98073-1-RR (same clone as 98073-1-PBS)

    1x10^6 human PBMCs were stained with APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC) and 0.25 ug Anti-Human IL-1RAP Rabbit Recombinant Antibody (98073-1-RR, Clone:240756A12), or Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98073-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98073-1-RR (same clone as 98073-1-PBS)

    FC experiment of human PBMCs using 98073-1-RR (same clone as 98073-1-PBS)

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human IL-1RAP Rabbit Recombinant Antibody (98073-1-RR, Clone:240756A12)(red), or Isotype Control(blue). Cells were not fixed. This data was developed using the same antibody clone with 98073-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00708-4

    Sandwich ELISA standard curve of MP00708-4

    Sandwich ELISA standard curve of MP00708-4, Human IL1RAP Recombinant Matched Antibody Pair - PBS only. 98073-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0827. 83726-5-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

  • Sample test of MP00708-4

    Sample test of MP00708-4

    Plasma of six individual healthy human donors was measured. The human IL1RAP concentration of detected samples was determined to be 266.79 pg/mL with a range of 13.79 - 1,302.80 pg/mL.

  • Sample test of MP00708-4

    Sample test of MP00708-4

    Serum of six individual healthy human donors was measured. The human IL1RAP concentration of detected samples was determined to be 21.67 pg/mL with a range of 7.89 - 42.37 pg/mL.

  • Affinity and Kinetic Characterization of 98073-1-RR

    Affinity and Kinetic Characterization of 98073-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98073-1-RR against Human IL-1RAP were performed. The affinity constant is 0.572 nM.

产品信息

98073-1-PBS targets IL-1RAP as part of a matched antibody pair:

MP00708-4: 98073-1-PBS capture and 83726-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 1 receptor accessory protein
Synonyms IL1RAP, IL 1RAcP, IL 1R3, IL 1R 3, EC:3.2.2.6
Calculated Molecular Weight65 kDa
GenBank Accession NumberNM_002182.4
Gene Symbol IL1RAP
Gene ID (NCBI) 3556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NPH3-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-1RAP (Interleukin-1 receptor accessory protein), also known as IL-1RAcP, IL-1R-3, or IL-1R3, is a member of the interleukin-1 receptor family. IL-1RAP is composed of an extracellular region containing three Ig-like C2 type domains, a transmembrane region, and a cytoplasmic region with a TIR (Toll-IL-1-receptor) domain. IL-1RAP functions as a co-receptor for IL-1, IL-33, IL-36. It plays an essential role in the signaling of the IL-1 family cytokines such as IL-1, IL-33, and IL-36, as well as tyrosine kinases FLT3 and c-Kit. IL-1RAP can also exist in a soluble form.