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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98083-1-RR

    FC experiment of human peripheral blood leukocytes using 98083-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7), or Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98083-1-RR

    FC experiment of human peripheral blood leukocytes using 98083-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7)(red), or 0.25 ug Isotype Control(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining.Cells were not fixed.

  • Affinity and Kinetic Characterization of 98083-1-RR

    Affinity and Kinetic Characterization of 98083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98083-1-RR against Human CLEC12A were performed. The affinity constant is 0.524 nM.

Anti-Human CLEC12A Rabbit Recombinant Antibody

CLEC12A Uni-rAbTM Recombinant Antibody for FC
Cat No. 98083-1-RR
CloneNo. 240791B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CLEC12A Ready-To-Use IHC Kit. CLEC12A primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98083-1-RR targets CLEC12A in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name C-type lectin domain family 12, member A
Synonyms CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1
Calculated Molecular Weight31 kDa
GenBank Accession NumberBC126291
Gene Symbol CLEC12A
Gene ID (NCBI) 160364
RRIDAB_3672230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5QGZ9
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).

实验方案

Product Specific Protocols
FC protocol for CLEC12A antibody 98083-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols