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Anti-Human CD23 Rabbit Recombinant Antibody

CD23 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241270C6

Cat no : 98088-1-RR

Print datasheet

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

验证数据展示

  • FC experiment of human PBMCs using 98088-1-RR

    FC experiment of human PBMCs using 98088-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98088-1-RR

    FC experiment of human PBMCs using 98088-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98088-1-RR

    Affinity and Kinetic Characterization of 98088-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98088-1-RR against Human CD23 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98088-1-RR targets CD23 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
Calculated Molecular Weight 321 aa, 36 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06734
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

实验方案

Product Specific Protocols
FC protocol for CD23 antibody 98088-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols