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Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody

IL3RA/CD123 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241268E3

Cat no : 98107-1-RR

Print datasheet

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

验证数据展示

  • FC experiment of human PBMCs using 98107-1-RR

    FC experiment of human PBMCs using 98107-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (98107-1-RR, Clone: 241268E3) or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98107-1-RR

    Affinity and Kinetic Characterization of 98107-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98107-1-RR against Human IL3RA/CD123 were performed. The affinity constant is 2.63 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98107-1-RR targets IL3RA/CD123 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 3 receptor, alpha (low affinity)
Synonyms IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra
Calculated Molecular Weight 378 aa, 43 kDa
GenBank Accession NumberBC035407
Gene Symbol CD123
Gene ID (NCBI) 3563
ENSEMBL Gene IDENSG00000185291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26951
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.

实验方案

Product Specific Protocols
FC protocol for IL3RA/CD123 antibody 98107-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols