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Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody

CTLA-4/CD152 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241100B8

Cat no : 98147-1-RR

Print datasheet

Synonyms

Ctla4, Ly 56, Ctla 4, Cd152, 241100B8

验证数据展示

  • FC experiment of mouse splenocytes using 98147-1-RR

    FC experiment of mouse splenocytes using 98147-1-RR

    1x10^6 untreated or Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed.

  • FC experiment of mouse splenocytes using 98147-1-RR

    FC experiment of mouse splenocytes using 98147-1-RR

    1x10^6 Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98147-1-RR

    Affinity and Kinetic Characterization of 98147-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98147-1-RR against Mouse CTLA-4/CD152 were performed. The affinity constant is 0.580 nM.

经过测试的应用

Positive FC detected inCon A treated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98147-1-RR targets CTLA-4/CD152 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytotoxic T-lymphocyte-associated protein 4
Synonyms Ctla4, Ly 56, Ctla 4, Cd152, 241100B8
Calculated Molecular Weight25 kDa
GenBank Accession NumberNM_009843.4
Gene Symbol CTLA-4
Gene ID (NCBI) 12477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09793
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

实验方案

Product Specific Protocols
FC protocol for CTLA-4/CD152 antibody 98147-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols