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验证数据展示

  • FC experiment of mouse bone marrow cells using 98197-1-RR

    FC experiment of mouse bone marrow cells using 98197-1-RR

    1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of mouse bone marrow cells using 98197-1-RR

    FC experiment of mouse bone marrow cells using 98197-1-RR

    1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98197-1-RR

    Affinity and Kinetic Characterization of 98197-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98197-1-RR against Mouse Neutrophil elastase were performed. The affinity constant is below 1 pM.

Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody

Neutrophil elastase Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98197-1-RR

产品说明书

CloneNo. 241842G3

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Leukocyte elastase, Elastase-2, Elane, Ela2, EC:3.4.21.37

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inmouse bone marrow cells

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98197-1-RR targets Neutrophil elastase in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 elastase 2, neutrophil
别名 Leukocyte elastase, Elastase-2, Elane, Ela2, EC:3.4.21.37
计算分子量29KD
GenBank蛋白编号NM_015779.2
基因名称 Ela2
Gene ID (NCBI) 50701
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ3UP87
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for Neutrophil elastase antibody 98197-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols