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验证数据展示

  • FC experiment of human PBMCs using 98260-1-RR

    FC experiment of human PBMCs using 98260-1-RR

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98260-1-RR

    FC experiment of human PBMCs using 98260-1-RR

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11)(red) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98260-1-RR

    Affinity and Kinetic Characterization of 98260-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98260-1-RR against Human EMR2/CD312 were performed. The affinity constant is 0.529 nM.

Anti-Human EMR2/CD312 Rabbit Recombinant Antibody

EMR2/CD312 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98260-1-RR

产品说明书

CloneNo. 242042B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

EMR2, EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98260-1-RR targets EMR2/CD312 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 egf-like module containing, mucin-like, hormone receptor-like 2
别名 EMR2, EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2
计算分子量90 kDa
GenBank蛋白编号NM_013447.3
基因名称 EMR2
Gene ID (NCBI) 30817
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHX3-1
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as CD312 or ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).

实验方案

Product Specific Protocols
FC protocol for EMR2/CD312 antibody 98260-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols