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Recombinant Human Complement factor H protein (rFc Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

3075

Accession

P08603-1

Cat No : Eg3753

Print datasheet

Synonyms

CFH,Complement factor H,ARMD4,ARMS1,Factor H

验证数据展示

  • Purity of Recombinant Human Complement factor H was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Complement factor H was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human Complement factor H protein Ser860-Arg1231 (Accession# P08603-1) with a rabbit IgG Fc tag at the C-terminus.
Predicted Molecular Mass 71.0 kDa
SDS-PAGE 75-95 kDa, reducing (R) condition
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).

References:

1.Ripoche, J. et al.(1988) Biochem J. 249(2):593-602.
2.Esparza-Gordillo, J. et al.(2004) Mol immunol. 41(4):355-67.
3.Pickering, MC. et al.(2008) Clin exp immunol. 151(2):210-30.