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GCSH Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-20 ng/mL

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00021-3

Print datasheet

Synonyms

GCE, GCSH, NKH

Validation Data Gallery

  • Cytometric bead array standard curve of MP00021-3, GCSH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83012-3-PBS. Detection antibody: 83012-2-PBS. Standard: Ag10174. Range: 0.625-20 ng/mL.

    Cytometric bead array standard curve of MP00021-3

    Cytometric bead array standard curve of MP00021-3, GCSH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83012-3-PBS. Detection antibody: 83012-2-PBS. Standard: Ag10174. Range: 0.625-20 ng/mL.

  • Sandwich ELISA standard curve of MP00021-3, Human GCSH Recombinant Matched Antibody Pair - PBS only. 83012-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10174. 83012-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

    Sandwich ELISA standard curve of MP00021-3

    Sandwich ELISA standard curve of MP00021-3, Human GCSH Recombinant Matched Antibody Pair - PBS only. 83012-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10174. 83012-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • The mean GCSH concentration was determined to be 24.39 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load and 23.77 ng/mL in HEK-293 cell extract based on a 3.40 mg/mL extract load.

    Sample test of MP00021-3

    The mean GCSH concentration was determined to be 24.39 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load and 23.77 ng/mL in HEK-293 cell extract based on a 3.40 mg/mL extract load.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00021-3 targets GCSH in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-20 ng/mL (Cytometric Bead Array); 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: GCSH Recombinant antibody, PBS Only (Capture) 83012-3-PBS (230505C10). 100 μg. Concentration 1 mgl/ml.

Detection antibody: GCSH Recombinant antibody, PBS Only (Capture/Detector) 83012-2-PBS (230505C4). 100 μg. Concentration 1 mgl/ml.

Alternative GCSH matched antibody pairs: MP00021-1, MP00021-2, MP50697-1, MP50697-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83012-3-PBS
Tested Reactivity human
Immunogen GCSH fusion protein Ag10174
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycine cleavage system protein H (aminomethyl carrier)
Synonyms NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE, 230505C10
GenBank accession numberBC000790
Gene symbol GCSH
UniprotP23434
Gene ID (NCBI) 2653
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83012-2-PBS
Tested Reactivity human
Immunogen GCSH fusion protein Ag10174
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycine cleavage system protein H (aminomethyl carrier)
Synonyms NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE, 230505C4
GenBank accession numberBC000790
Gene symbol GCSH
UniprotP23434
Gene ID (NCBI) 2653
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C