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NSUN2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/ml

Cat No : MP00112-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00112-3, Human NSUN2 Recombinant Matched Antibody Pair - PBS only. 82894-9-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14791. 82894-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/ml

    Sandwich ELISA standard curve of MP00112-3

    Sandwich ELISA standard curve of MP00112-3, Human NSUN2 Recombinant Matched Antibody Pair - PBS only. 82894-9-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14791. 82894-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/ml

  • HCT-116 cell lysate was measured. The human NSUN2 concentration of detected samples was determined to be 15,374.1 pg/mL (based on a 3.0 mg/mL extract load).

    Sample test of MP00112-3

    HCT-116 cell lysate was measured. The human NSUN2 concentration of detected samples was determined to be 15,374.1 pg/mL (based on a 3.0 mg/mL extract load).

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00112-3 targets NSUN2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/ml (Sandwich ELISA);

Capture antibody: NSUN2 Recombinant antibody, PBS Only (Capture) 82894-9-PBS (230186D9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: NSUN2 Recombinant antibody, PBS Only (Detector) 82894-1-PBS (230186A6). 100 μg. Concentration 1 mgl/ml.

Alternative NSUN2 matched antibody pairs: MP00112-1, MP00112-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 82894-9-PBS
Tested Reactivity human
Immunogen NSUN2 fusion protein Ag14791
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-
GenBank accession numberBC001041
Gene symbol NSUN2
UniprotQ08J23
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 82894-1-PBS
Tested Reactivity human
Immunogen NSUN2 fusion protein Ag14791
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-
GenBank accession numberBC001041
Gene symbol NSUN2
UniprotQ08J23
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C