• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NSUN2 Recombinant antibody, PBS Only (Detector)

NSUN2 Recombinant Antibody for IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230186A6

Cat No : 82894-1-PBS

Print datasheet

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

验证数据展示

  • IF Staining of HepG2 using 82894-1-RR (same clone as 82894-1-PBS)

    IF Staining of HepG2 using 82894-1-RR (same clone as 82894-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-1-RR, Clone: 230186A6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00112-1

    Cytometric bead array standard curve of MP00112-1

    Cytometric bead array standard curve ofMP00112-1, NSUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82894-4-PBS. Detection antibody: 82894-1-PBS. Standard: Ag14791. Range: 0.625-20 ng/mL.

82894-1-RR

Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

产品信息

82894-1-PBS targets NSUN2 as part of a matched antibody pair:

MP00112-1: 82894-4-PBS capture and 82894-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen NSUN2 fusion protein Ag14791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4
Calculated Molecular Weight 767 aa, 86 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08J23
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.