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验证数据展示

  • Sandwich ELISA standard curve of MP00112-3

    Sandwich ELISA standard curve of MP00112-3

    Sandwich ELISA standard curve of MP00112-3, Human NSUN2 Recombinant Matched Antibody Pair - PBS only. 82894-9-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14791. 82894-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/ml

  • Sample test of MP00112-3

    Sample test of MP00112-3

    HCT-116 cell lysate was measured. The human NSUN2 concentration of detected samples was determined to be 15,374.1 pg/mL (based on a 3.0 mg/mL extract load).

  • IF Staining of HepG2 using 82894-1-RR (same clone as 82894-1-PBS)

    IF Staining of HepG2 using 82894-1-RR (same clone as 82894-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-1-RR, Clone: 230186A6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82894-1-RR (same clone as 82894-1-PBS)

    FC experiment of HeLa using 82894-1-RR (same clone as 82894-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human NSUN2 (82894-1-RR, Clone:230186A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00112-1

    Cytometric bead array standard curve of MP00112-1

    Cytometric bead array standard curve of MP00112-1, NSUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82894-4-PBS. Detection antibody: 82894-1-PBS. Standard: Ag14791. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 82894-1-RR

    Affinity and Kinetic Characterization of 82894-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82894-1-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

NSUN2 Recombinant antibody, PBS Only (Detector)

NSUN2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 82894-1-PBS

产品说明书

CloneNo. 230186A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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82894-1-RR

Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

产品信息

82894-1-PBS targets NSUN2 as part of a matched antibody pair:

MP00112-1: 82894-4-PBS capture and 82894-1-PBS detection (validated in Cytometric bead array)

MP00112-3: 82894-9-PBS capture and 82894-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 NSUN2 fusion protein Ag14791 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 2
别名 RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-
计算分子量 767 aa, 86 kDa
GenBank蛋白编号BC001041
基因名称 NSUN2
Gene ID (NCBI) 54888
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ08J23
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.